Journal: bioRxiv

Article Title: De novo protein design enables precise induction of functional antibodies in vivo

doi: 10.1101/685867

Figure Lengend Snippet: A) Multiple sequence alignment of S4_2 sequences that were enriched after yeast screening for protease resistance and binding to 101F. Sequences are colored according to a BLOSUM62 score matrix. B) Circular dichroism spectra for 12 designs of the S4_2 design series that were enriched for protease resistance and binding to 101F in the yeast display selection assay. Black: spectrum at 20 °C. Red: spectrum at 90 °C. C) SPR sensorgrams for binding to 101F for the same designs. 101F IgG was immobilized on the CM5 sensor chip surface, and the designs were flown as analyte.

Article Snippet: Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988) via amine coupling.

Techniques: Sequencing, Binding Assay, Selection

Journal: Cell reports

Article Title: Targeting the Oncogenic Long Non-coding RNA SLNCR1 by Blocking Its Sequence-Specific Binding to the Androgen Receptor

doi: 10.1016/j.celrep.2019.12.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 2015 Nov;10(11):1643–69. https://doi.org/10.1038/nprot.2015.103 http://weeks.chem.unc.edu/software.html MEME Suite Bailey et al., 2009 http://meme-suite.org GraphPad Prism version 7.01 for Windows GraphPad Software https://www.graphpad.com/ Other 5% Mini-PROTEAN® TBE Gel, 10 well, 30 μl BioRad Cat456501S Series S Sensor Chip CM5, pack of 1 GE Healthcare Cat#29104988 Corning BioCoat Matrigel Invasion Chamber with Corning Matrigel Matrix Fisher Scientific Cat# {"type":"entrez-protein","attrs":{"text":"S54480","term_id":"1084872","term_text":"pir||S54480"}} S54480 Open in a separate window KEY RESOURCES TABLE The N-terminal region of AR binds unstructured RNA in a sequence-specific manner An unstructured lncRNA region scaffolds an invasion-promoting transcription complex An identified AR RNA-binding motif is predictive of other lncRNA-AR interactions Sterically blocking the SLNCR -AR interaction blocks melanoma invasion

Techniques: Recombinant, Reverse Transcription, Transfection, cDNA Synthesis, Sequencing, Generated, Software

Journal: bioRxiv

Article Title: ARGX-119, a therapeutic agonist antibody targeting MuSK

doi: 10.1101/2024.07.18.604166

Figure Lengend Snippet: (A) Binding of ARGX-119 or Ig1-like domain binding antibody control (3F6c-hIgG1-LALAdelK) to MuSK-ECD or MuSK Fz-like domain on ELISA. (B) SPR binding of ARGX-119 to MuSK. CM5 chip was coated with anti-human IgG-Fc and ARGX-119 was captured at low densities. Sensorgrams show the binding kinetics of 10 nM human, NHP, mouse and rat MuSK using a dissociation pH of 7.4 or 5.5. Kinetic parameters (C) ITC experiment, investigating the binding thermodynamics of ARGX-119 to human, NHP, mouse and rat MuSK in solution.

Article Snippet: Goat anti-human IgG antibodies and the Fcγ fragment specific (109-005-098, Jackson Immunoresearch) were covalently immobilized onto all the flow cells of a Sensor Chip CM5 Series S (Cytiva) at high density using amine coupling chemistry.

Techniques: Binding Assay, Control, Enzyme-linked Immunosorbent Assay